Post-doctoral Research Scientist in Buffa's Laboratory

IFOM The FIRC Institute of Molecular Oncology Foundation

Milan, Italy

The Buffa Lab is seeking a highly motivated postdoctoral scientist to work on a project entitled “Single-cell AI-reconstructed gene network rewiring in hypoxia to dissect mechanisms of cancer progression and metastasis (REWIRE)”.

The laboratory uses systems biology approaches to understand how cancer evolves and responds to treatment, and how the tumor microenvironment affects this process. In our previous studies, we have extensively characterized the transcriptional response to hypoxia, in bulk and single cells, in multiple cancer types.

This project aims to further understand the reprogramming of cells exposed to early and chronic hypoxia in multiple experimental models, with a particular focus on CRISPR and single cell sequencing. The project will involve perturbation experiments in cell lines, organoids and single cell next generation sequencing approaches.

Profile Description

The ideal candidate will possess robust communication skills, self-motivation, and the ability to work autonomously. Additionally, they should be capable of fostering collaboration and constructive contributions within IFOM's scientific environment.

• Proficiency in advanced biochemical, molecular and cell biology techniques;
• Demonstrable knowledge and experience of current omics and CRISPR assays;
• Publications in internationally recognized journals;
• Presentations at conferences, seminars or other relevant meetings;
• Ability to work independently and as part of a team;
• Ability to lead and manage small research projects;
• Attention to detail and accurate data management;
• Reliability and flexibility.

Language Skills:

• Proficiency in English for speaking and writing. English is the working language at the Institute.

Required Skills:

• PhD or equivalent degree in Molecular Biology, Genetics, Biotechnology or related field;
• Research experience in genomics, functional genomics and perturbation-based assays;
• Advanced molecular biology techniques;
• Experience with CRISPR-based screens;
• Experience with transcriptomics, single-cell genomics or proteomics;
• Proven publication record in peer-reviewed journals;
• Excellent communication skills.

Additionally Desired Skills:

• First-author publications in internationally recognised journals;
• Previous grant funding;
• Experience with other biochemical techniques (e.g. shRNA).